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Specifically, we observed that 212 elements (181 unique genes) and 60 elements (47 unique genes) were upregulated no less than twofold by H RasV12 and Rit79L, respectively.
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OPN is often a secreted adhesive glycoportein with diverse functions, which is implicated in cancer development, progression and metastasis (Oates et al.
We identified downstream signaling events and transcriptional targets with the OPN CD44 autocrine loop.
G418 selected cells expressing H RasV12, Rit79L, RhoA QL, Rac1 QL or Cdc42 QL were based upon Western blot analysis expressing comparable levels of the mutant GTPases (data not shown).
Foci formation was greatest in H RasV12, intermediate in Rit79L and lowest in RhoA QL, Rac1 QL and Cdc42 QL transformed cells (Figure 1a).
The graded transformation response all over the entire panel with the mutant GTPases was critical in order to correlate foci formation with gene expression changes.
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