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, NJ, USA) using goat anti mouse or goat anti rabbit IgGs coupled to horseradish peroxidase for a secondary antibody (Santa Cruz, CA, USA and Cappel, NC, USA respectively).
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As a consequence of in order to detect endogenous Sec16B, further characterization of Sec16B was performed using GFP Sec16B.
"It needs some type of surprise, a bombshell," Lamkin said.
Proc Natl Acad Sci U S A 97, 11307–.
Specificity of the antibodies, however, was proven by detection in excess of expressed GFP Sec16B.
It is additionally quite likely that the expression of Sec16B is just regulated.
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