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Full size image View in article Figure 3: Depletion of Sec16B in HeLa cells does not affect the localization of Sec24C or ERGIC 53, nor can GFP Sec16B functionally replace Sec16A.
Thus, either those cell types examined will not express Sec16B, or the antibodies usually are not of sufficient avidity to detect an exceptionally low-level of endogenous expression of Sec16B.
RPE1 cells stably expressing GFP Sec16B were transfected with siRNA duplexes targeting Sec16B (Sec16B “.
Total RNA (15 g total RNA) was adopted to synthesize fluorescent cDNA target by reverse transcription with random primers and aminoallyl dUTP with chemical coupling with the Cy5 or Cy3 ester.
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