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The three antibodies detected GFP Sec16B (A, B and C lane 1 #).
(C) Following methanol fixation, GFP tagged tER localization constructs were primarily cytosolic and also the nuclear pool was mainly extracted.
Hence, we initiated the very first global check out downstream transcriptional targets of OPN.
We generated and affinity purified three different anti peptide antibodies, anti Sec16B (996–.
No endogenous Sec16B could be detected in any of the cell types.
Knockdown of OPN expression in H RasV12 transformed NIH3T3 cells ended in significant temporal adjustments to gene expression.
The bands at ~ 100 kD identified by anti Sec16B (883–.
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