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as used in21, Sec16B UTR working out . 3′.
untranslated region, Sec16B #1 and Sec16B #2 working out . coding sequence).
No endogenous Sec16B can be detected in any of the cell types.
HeLa and RPE 1 cells were subsequently transduced together with the virus and stably expressing cells were selected with puromycin.
Cells were observed under an inverted fluorescent microscope (Leica), and photographs were taken having a Leica DFC 500 camera.
Total RNA from cell lines were reversed transcribed using random primers depending on the manufacturer's protocol.
Immunoblotting having an anti GFP antibody says in the cell lines GFP Sec16B was expressed to be a correct fusion protein creating a molecular weight of ~ 144 kD (data not shown).
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And until we stop putting our faith, and our dollars, into those hands, we shall continue in this particular whirlpool of debt.
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The action will likely be Saudi Arabia's first since May and yes it comes into this match for the back of losses to Moldova and Georgia.
Saudi Arabia has won one among its five previous matches against Australia though five straight defeats and no win in seven themselves, Luongo said Saudi Arabia would prove a tricky opponent.
I feel it is a good possibility to get confidence up, said Luongo, who would not have any game time in the World Cup in Brazil despite finding yourself in the squad.
We now have played against several of the top teams on the earth already.
After we will take everything we do for the training ground into games against several of the what is known as lesser teams you will need to is going to do OK.
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