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Because OAT3 is exclusively expressed at endothelial cells during the brain, we might evaluate OAT3 mRNA levels in BCECs by analyzing these levels during the entire brain.
Thus, no actual particular feature of ribosome binding site with the trpE gene, but attenuator structure itself is in charge of the observed effect.
SRSF3 protein with N or C terminal FLAG tag was expressed in HEK293T cells.
Really the only His misincorportation detected during the Cat protein during the three strains was at the Trp16 position during the tryptic peptide ITGYTTVDISQWHR.
To measure and compare His misincorporation into proteins of suppressed cells, we created a new quantitative assay for proteome analysis according to mass spectrometry.
In strains G2141 and G2220 (strains isolated after forced evolution), 16 and 31 His misincorporations were identified in 12 and 20 different cellular proteins respectively (table 4).
Full size image View in article Figure 4: Localization of GFP Sec16B compared to other ERES and ERGIC components.
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