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Selection stringency can be tuned by modifying the ratio of the sums of the canonical and miscoding aminoacyl tRNAs recognizing the Zaa codon.
Essentially the most favorable cases match substituting all 3 bases of any codon such as CUU (leucine) for AAA or AAG (lysine) and GCG (alanine) for UGC (cysteine).
ChemPort ISI PubMed McCarthy, A.
Observe that aspartate will not be hyposteric to glutamate as the common carboxylate moieties may not be superimposable.
Selection stringency can be tuned by modifying the ratio of the sums of the canonical and miscoding aminoacyl tRNAs recognizing the Zaa codon.
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