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Tryptophan, encoded with a single codon UGG generally in most organisms, would be the rarest protein from the proteome of E.
We describe the identification associated with an essential histidine inside an essential protein.
We replaced the codon due to this histidine with a tryptophan codon, and transformed cellular matrix with a construct encoding a suppressor tRNA (recognizing the Trp codon and incorpotating a His residue).
PCR analysis while using the primer pairs shown in (e).
We replaced the codon due to this histidine with a tryptophan codon, and transformed cellular matrix with a construct encoding a suppressor tRNA (recognizing the Trp codon and incorpotating a His residue).
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